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antigenic epitope alpha gal  (Vector Laboratories)


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    Vector Laboratories antigenic epitope alpha gal
    Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal <t>epitope</t> and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)
    Antigenic Epitope Alpha Gal, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigenic epitope alpha gal/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    antigenic epitope alpha gal - by Bioz Stars, 2026-03
    86/100 stars

    Images

    1) Product Images from "The fabrication and characterization of a multi-laminate, angle-ply collagen patch for annulus fibrosus repair"

    Article Title: The fabrication and characterization of a multi-laminate, angle-ply collagen patch for annulus fibrosus repair

    Journal: Journal of tissue engineering and regenerative medicine

    doi: 10.1002/term.2250

    Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal epitope and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)
    Figure Legend Snippet: Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal epitope and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)

    Techniques Used: Staining, Immunohistochemistry, Isolation, Spectrophotometry, Light Microscopy



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    antigenic epitope alpha gal  (Vector Laboratories)
    86
    Vector Laboratories antigenic epitope alpha gal
    Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal <t>epitope</t> and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)
    Antigenic Epitope Alpha Gal, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigenic epitope alpha gal/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    antigenic epitope alpha gal - by Bioz Stars, 2026-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal epitope and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)

    Journal: Journal of tissue engineering and regenerative medicine

    Article Title: The fabrication and characterization of a multi-laminate, angle-ply collagen patch for annulus fibrosus repair

    doi: 10.1002/term.2250

    Figure Lengend Snippet: Representative histological images of (A) fresh and (B) decellularized porcine pericardium stained with haematoxylin and eosin (H&E), respectively (pink = extracellular matrix; arrowheads = location of cell nuclei; total magnification: 200 ×). Representative histological images of (C) fresh porcine pericardium demonstrating positive (brown) immunohistochemistry (IHC) staining for alphagal epitope and (D) decellularized pericardium illustrating a lack of positive staining (blue = matrix; black = cell nuclei; total magnification: 200 ×; inserts = negative IHC controls). (E) Ethidium bromide-stained agarose gels for DNA isolated from fresh (lanes 1–5) and decellularized (lanes 6–10) pericardium (white bands = presence of DNA). A 300–24 000-bp DNA standard ladder (lanes 11–12) is shown for comparison. (F) DNA quantification of fresh and decellularized pericardium performed with Nanodrop spectrophotometry. (G) Diagrammatic representation of multi-laminate annulus fibrosus (AF) patch formation in which at least three-plys of decellularized pericardium were stacked (red tubes = aligned collagen type I fibres in the fibrous pericardium layer of each ply) oriented at ±30° to each other. (H) Schematic representation depicting histology sectioning of AF patches using an oblique cut (dotted red line) across multiple layers performed to microscopically visualize collagen fibre alignment in fibrous pericardium surfaces stacked directly adjacent to one another demonstrating a ±30° ‘chevron’ (*) pattern. Alternatively, when fibrous and parietal pericardium surfaces were directly adjacent to each other, a ‘half chevron’ (#) was achieved. (I) Chevron and (J) half chevron patterns (dashed white outlines), respectively, were observed within each patch via polarized light microscopy confirming the presence of oriented collagen fibre alignment (total magnification: 100 ×). (K) Macroscopic image of a six-ply AF patch sewn with suture (black outline). Solid lines connecting different study groups on the graph indicate a significant difference (p < 0.05)

    Article Snippet: Additionally, immunohistochemistry (IHC) for the porcine antigenic epitope alpha-gal (biotinylated GSL1-isolectin B4; 2.5 μg/ml; VectorLabs) was performed.

    Techniques: Staining, Immunohistochemistry, Isolation, Spectrophotometry, Light Microscopy